First, I needed to make some agar up
Bring around 500ml of water to the boil
Add enough dried malt extract (DME) to produce a s.g. of around 1040-1045.
Add in 1/2 capsule of Servomyces yeast nutrient
Boil for 10 minutes.
Filter out the hot break
Add 7gm of agar agar and boil for 2 minutes
Split into slant tubes, between 15ml and 25ml/tube
When the vent starts steaming throw on the valve and leave under pressure for 10 minutes
Leave to cool, release pressure and lay out tubes on a slant to produce a large surface area for yeast inoculation
Take your mother yeast colony - in the case White Labs Brettanomyces Bruxellensis Trois (WLP644) Belgian Ale Yeast (on the left, below), a slant and a sterile needle (here a single use tattoo needle, EO sterilised)